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DNA聚合酶、显色剂、标准阳性模板以及dd H2O。本发明的试剂盒具有敏感性高、特异性强、反应迅速、操作简单等特点，不需要特殊的仪器设备，解决了弓形虫病检测时间长、工作量大、操作复杂等缺陷。可以用于检测临床样本是否携带弓形虫，以及弓形虫病的早期诊断和分子流行病学调查。","2011.02.16#公开;2011.03.30#实质审查的生效;2012.08.01#授权","CN201010526397.X","C12Q1\u002F68","张德林;王萌;王艳华;付宝权;李文卉",[],[],{"resultType":386,"id":467,"title":468,"abstract":469,"orgName":417,"province":391,"date":418,"trl":10,"transferMode":10,"keywords":10,"industry":10,"patentType":393,"legalStatus":470,"isOpenLicense":395,"isRevitalized":396,"techMaturity":10,"starRating":10,"licenseFeeText":10,"patentNo":471,"ipc":472,"inventors":473,"tags":474,"tagNames":475},"gpi_01927","一种检测羊无浆体病原的环介导恒温扩增方法","本发明公开一种用于检测羊无浆体病原的环介导恒温扩增(LAMP)方法。以羊无浆体主要表面蛋白4基因为靶基因，设计了3对特异性引物，并按一定比例配制成特异性引物反应混合物，在恒温条件下进行DNA扩增。不需要复杂的仪器如PCR扩增仪，只需要常规的水浴锅就可以完成检测，并具有快速、敏感、高特异性和操作简单的特点，在常规的实验室就可以进行该病原的检测。因此，其适合于羊无浆体病原的检测和大规模的流行病学调查，而且可得到具有高敏感性的检测结果。","2011.03.16#公开;2011.05.04#实质审查的生效;2012.09.26#著录事项变更;2012.10.17#授权","CN201010542863.3","C12Q1\u002F68;C12Q1\u002F04","殷宏;罗建勋;马朱玲;刘志杰;杨志飞;关贵全;李有爱;刘爱红;任乔云;刘军龙;牛庆丽",[],[],{"resultType":386,"id":477,"title":478,"abstract":479,"orgName":480,"province":391,"date":418,"trl":10,"transferMode":10,"keywords":10,"industry":10,"patentType":393,"legalStatus":481,"isOpenLicense":395,"isRevitalized":396,"techMaturity":10,"starRating":10,"licenseFeeText":10,"patentNo":482,"ipc":483,"inventors":484,"tags":485,"tagNames":486},"gpi_01017","一种治疗乳腺增生病的中成药及其制备工艺","本发明涉及一种治疗乳腺增生病的中成药，该方药由下述原料重量份组成：柴胡10～30、当归10～30、赤芍10～30、丹参15～45、橘核9～27、王不留行9～27、海藻10～30、昆布10～30、制香附9～27、制没药5～20、制乳香5～20、鹿角霜15～40，以理气、活血、止痛、软坚、散结、调补冲任立法，达到标本兼治之功效。同时本发明还公开了该方药的制备方法。","甘肃省医学科学研究院","2010.02.10#公开;2010.04.14#实质审查的生效;2011.02.16#授权","CN200910117431.5","A61K36\u002F8905;A61P15\u002F14;A61K35\u002F32","石晓峰;范彬;沈薇;张芳红;马趣环;刘元强",[],[],{"resultType":386,"id":488,"title":489,"abstract":490,"orgName":417,"province":391,"date":418,"trl":10,"transferMode":10,"keywords":10,"industry":10,"patentType":393,"legalStatus":491,"isOpenLicense":395,"isRevitalized":396,"techMaturity":10,"starRating":10,"licenseFeeText":10,"patentNo":492,"ipc":493,"inventors":494,"tags":495,"tagNames":496},"gpi_01829","一种猪口蹄疫苗用的复合分子型佐剂及其制造方法","本发明涉及一种猪口蹄疫苗用的、由CpG基序和猪IFN-γ基因克隆入真核表达载体构成的佐剂及其制造方法，以及猪口蹄疫病毒灭活抗原疫苗与这种佐剂配伍而成猪口蹄疫苗组合物。本发明的这种复合分子型佐剂，其CpG基序为至少由3个GTCGTT、2个GACGTT和1个AACGTT特殊序列构成的一段基序，其序列为SEQ No：1，用其构建的PcDNA3.1重组质粒，并在大肠杆菌中增殖。","2011.01.26#公开;2011.03.23#实质审查的生效;2013.11.06#授权","CN201010297461.1","A61K39\u002F39;A61K39\u002F135;C12N15\u002F85;C12N15\u002F117;A61P31\u002F14","景志忠;陈国华;房永祥;窦永喜;蒙学莲;赵娜;曾爽;贾怀杰;才学鹏",[],[],{"resultType":386,"id":498,"title":499,"abstract":500,"orgName":417,"province":391,"date":418,"trl":10,"transferMode":10,"keywords":10,"industry":10,"patentType":393,"legalStatus":501,"isOpenLicense":395,"isRevitalized":396,"techMaturity":10,"starRating":10,"licenseFeeText":10,"patentNo":502,"ipc":503,"inventors":504,"tags":505,"tagNames":506},"gpi_00375","一种猪囊尾蚴病间接ELISA检测试剂盒及其制备方法","本发明提供了一种检测猪囊尾蚴抗体的间接ELISA试剂盒及其制备方法与应用。该试剂盒包括以基因工程表达的猪囊尾蚴TS－CC18蛋白作为包被抗原的ELISA板条，PBST洗液，血清稀释液，辣根过氧化物酶标记的兔抗猪IgG、显色底物、终止液及猪囊尾蚴标准阴阳性血清等。所用的重组抗原的制备是将编码猪囊尾蚴TS－CC18基因的片断与表达载体PET28a(+)连接，并转化大肠杆菌BL21(DE3)进行诱导表达；表达蛋白经镍柱亲和纯化后包被酶标板。本发明的优势体现在具有良好的特异性和敏感性、检测成本低廉、操作简单、可大量生产以及生产周期短等优点，有着良好的推广应用价值。","2008.11.12#公开;2009.01.07#实质审查的生效;2012.06.27#授权","CN200810108465.3","G01N33\u002F53","才学鹏;张少华;景志忠;骆学农;郭爱疆;郑亚东;贾万忠;窦永喜",[],[],{"resultType":386,"id":508,"title":509,"abstract":510,"orgName":511,"province":391,"date":418,"trl":10,"transferMode":10,"keywords":10,"industry":10,"patentType":393,"legalStatus":512,"isOpenLicense":395,"isRevitalized":396,"techMaturity":10,"starRating":10,"licenseFeeText":10,"patentNo":513,"ipc":514,"inventors":515,"tags":516,"tagNames":517},"gpi_00849","一种生长抑素的合成方法","本发明涉及生长抑素的一种液相合成方法，其合成方法为：首先分别全合成Thr(Bzl)－Phe－Thr(Bzl)－Ser(Bzl)－Cys(MeOBzl)－OBzl、Boc－Phe－Trp－Lys(Z)－OH、Boc－Asn－Phe－OH和Boc－Ala－Gly－Cys(MeOBzl)－Lys(Z)－OH四4个片段肽，再用EDCI\u002FHOBt进行片段缩合，合成全保护的还原性十四肽，用三氟甲磺酸和苯甲醚系统脱肽链所有的保护基，得到还原性生长抑素，对还原性生长抑素氧化，获得生长抑素粗品，再经C18制备柱分离纯化，冷冻干燥后，得生长抑素。","兰州大学;甘肃中科药源生物工程股份有限公司","2009.11.04#公开;2012.05.09#实质审查的生效;2013.07.10#授权","CN200910142420.2","C07K7\u002F08;C07K1\u002F06;C07K1\u002F02;A61P5\u002F02;A61P5\u002F08;A61P5\u002F48;A61P1\u002F04;A61P1\u002F18;A61P7\u002F04","达朝山;苏亚宁;洪满贵;保英明;曹福军",[],[],{"resultType":386,"id":519,"title":520,"abstract":521,"orgName":417,"province":391,"date":418,"trl":10,"transferMode":10,"keywords":10,"industry":10,"patentType":393,"legalStatus":522,"isOpenLicense":395,"isRevitalized":396,"techMaturity":10,"starRating":10,"licenseFeeText":10,"patentNo":523,"ipc":524,"inventors":525,"tags":526,"tagNames":527},"gpi_00644","一种用于检测猪繁殖与呼吸综合症病毒的试剂盒和检测方法","一种用于检测猪繁殖与呼吸综合症病毒的试剂盒和检测方法，所述试剂盒包含RNA酶抑制剂、AMV逆转录酶、Taq酶、无RNA酶水、One Step RNA PCR混合液、阴性对照以及序列SEQ ID NO：1至SEQ ID NO：6引物的混合物，所述检测包括使用所述试剂盒进行RT－PCR扩增的步骤。所述方法能快速、敏感、准确以及低成本地检测猪繁殖与呼吸综合征病毒。","2009.06.24#公开;2009.08.19#实质审查的生效;2013.02.20#授权","CN200810241706.1","C12Q1\u002F70;C12Q1\u002F68;C12N15\u002F11","刘湘涛;田宏;吴锦艳;尚佑军;尹双辉;靳野",[],[],{"resultType":386,"id":529,"title":530,"abstract":531,"orgName":532,"province":391,"date":418,"trl":10,"transferMode":10,"keywords":10,"industry":10,"patentType":393,"legalStatus":533,"isOpenLicense":395,"isRevitalized":396,"techMaturity":10,"starRating":10,"licenseFeeText":10,"patentNo":534,"ipc":535,"inventors":536,"tags":537,"tagNames":538},"gpi_01938","一种菜籽油用咪唑盐类极压抗磨添加剂及其制备方法","本发明公开了一种菜籽油用咪唑盐类极压抗磨添加剂及其制备方法。添加剂的化学名称为二烷基二硫代磷酸咪唑盐离子液体。本发明以三氯甲烷作溶剂，将二烷基二硫代磷酸以及二烷基咪唑溴盐混合均匀，常温搅拌反应，然后用蒸馏水洗至水层为中性，除去溶剂，得到产物为浅黄色粘稠液体。添加剂具有较高热稳定性和优异的减摩抗磨性能，可以作为菜籽油添加剂使用。","中国科学院兰州化学物理研究所","2011.03.23#公开;2011.05.04#实质审查的生效;2012.12.12#授权","CN200910117411.8","C10M137\u002F10;C10M133\u002F46;C07F9\u002F17;C07D233\u002F58;C10N30\u002F06","刘维民;李金龙;周峰;夏延秋",[],[],{"resultType":386,"id":540,"title":541,"abstract":542,"orgName":543,"province":391,"date":418,"trl":10,"transferMode":10,"keywords":10,"industry":10,"patentType":393,"legalStatus":544,"isOpenLicense":395,"isRevitalized":396,"techMaturity":10,"starRating":10,"licenseFeeText":10,"patentNo":545,"ipc":546,"inventors":547,"tags":548,"tagNames":549},"gpi_00872","一种锁阳多糖的制备方法","本发明公开了一种锁阳多糖的制备方法，包括如下步骤：在锁阳粉末中加入蒸馏水充分搅匀，浸泡24－36h后，补入蒸馏水，使料液比达到1\u002F26－1\u002F10，再进行超声波萃取，得到反应液；将所述反应液移至微波反应釜中，辅以机械搅拌，在40－80℃的温度条件、以及100－500W的功率条件下，反应20－60min后，进行过滤，得到上清液；对所述上清液依次进行浓缩处理、醇沉处理和离心处理，得到下沉沉淀，再对所述沉淀进行真空冷冻干燥处理，得到所述锁阳多糖。本发明所述制备方法，相比传统水提法，具有产率高、提取时间短和节约能耗的优点。","西北师范大学","2009.11.18#公开;2010.01.13#实质审查的生效;2012.03.21#授权","CN200910148024.0","C08B37\u002F00;A61P37\u002F04;A61P39\u002F06","张继;王风霞;刘忠旺;舒何晶;张兴晶;杨玉萍;张玉媛;徐小龙;梁俊玉;马君义;姚健",[],[],{"resultType":386,"id":551,"title":552,"abstract":553,"orgName":554,"province":391,"date":418,"trl":10,"transferMode":10,"keywords":10,"industry":10,"patentType":393,"legalStatus":555,"isOpenLicense":395,"isRevitalized":396,"techMaturity":10,"starRating":10,"licenseFeeText":10,"patentNo":556,"ipc":557,"inventors":558,"tags":559,"tagNames":560},"gpi_01140","一种防治畜禽温热病药物组合物及其制备方法","本发明公开一种防治畜禽温热病药物组合物及其制备方法。以1500ml药液计算，药物含各味中药以生药材计算的量以及敷料量的配比为：连翘350～450g，板兰根150～250g，金银花150～250g，黄芩150～250g，黄连150～250g，鸭趾草150～250g，甘草75～125g，苯甲醇2-3g，其余为蒸馏水。药物组合物制备方法是采用水提、醇沉、药液配制三步骤制成注射剂。本发明通过大量的临床使用，证明该药物具有抗病毒、抗菌、消炎、清热解毒、增强免疫力功能，对以发热为主要特征的温病具有十分良好的治疗效果，其应用对于推动我国畜禽业的发展将产生积极作用。","中国农业科学院兰州畜牧与兽药研究所","2010.05.05#公开;2010.06.23#实质审查的生效;2011.11.30#授权","CN200910117622.1","A61K36\u002F718;A61P29\u002F00;A61P31\u002F04;A61P31\u002F14;A61P31\u002F16;A61P37\u002F04","李剑勇;张继瑜;李新圃;杨亚军;周绪正;牛建荣;魏小娟;李冰;陈佳娟",[],[],{"resultType":386,"id":562,"title":563,"abstract":564,"orgName":543,"province":391,"date":418,"trl":10,"transferMode":10,"keywords":10,"industry":10,"patentType":393,"legalStatus":565,"isOpenLicense":395,"isRevitalized":396,"techMaturity":10,"starRating":10,"licenseFeeText":10,"patentNo":566,"ipc":567,"inventors":568,"tags":569,"tagNames":570},"gpi_00233","一种降低血糖产品的制备方法","一种降低血糖产品的制备方法；该方法是以锁阳为原料，通过粉碎、回流、抽滤、离心、酶解、萃取、减压浓缩、调节pH值、透析袋透析和离心等步骤，制备出一种主要用于治疗链脲佐菌素(STZ)造成的化学性糖尿病，并对糖尿病引起的肝脏、肾脏损伤具有明显修复作用的治疗药物产品。本发明充分利用甘肃、青海、内蒙古自治区境内遍布的寄生于蒺藜科白刺属植物的锁阳，且蕴藏量十分充足这一良好的资源优势，开发本产品，市场前景十分看好，经济效益广阔。","2008.07.16#公开;2008.09.10#实质审查的生效;2010.12.08#授权","CN200710187929.X","A61K36\u002F185;A61P3\u002F10","张继;吴依茜;王云普;王春霞;王俊龙;张亚楠;赵保堂",[],[],{"resultType":386,"id":572,"title":573,"abstract":574,"orgName":554,"province":391,"date":418,"trl":10,"transferMode":10,"keywords":10,"industry":10,"patentType":393,"legalStatus":575,"isOpenLicense":395,"isRevitalized":396,"techMaturity":10,"starRating":10,"licenseFeeText":10,"patentNo":576,"ipc":577,"inventors":578,"tags":579,"tagNames":580},"gpi_00325","丁香酚阿斯匹林酯药用化合物及其制剂和制备方法","本发明公开一种丁香酚阿斯匹林酯药用化合物，结构如(I)式所示。它具有药理作用更为明显，结构更为稳定的特点。它是以阿斯匹林为原料，经酰氯化，在溶媒中进行酯化反应，经重结晶制得的化合物。以丁香酚阿斯匹林酯药用化合物为有效药用成分，可制成用于人医和兽医临床上治疗各种高热病症，尤其是病毒性感染所致、预防血栓形成、皮肤真菌感染的固体制剂、液体制剂、注射剂、眼用制剂、软膏剂、栓剂、膜剂、气雾剂或外用制剂。","2008.09.24#公开;2008.11.19#实质审查的生效;2010.06.09#授权","CN200810017950.X","C07C69\u002F86;C07C67\u002F08;A61K31\u002F621;A61P29\u002F00;A61P31\u002F00;A61P7\u002F02","李剑勇;张继瑜;周绪正;牛建荣;魏小娟",[],[],{"resultType":386,"id":582,"title":583,"abstract":584,"orgName":585,"province":391,"date":418,"trl":10,"transferMode":10,"keywords":10,"industry":10,"patentType":393,"legalStatus":586,"isOpenLicense":395,"isRevitalized":396,"techMaturity":10,"starRating":10,"licenseFeeText":10,"patentNo":587,"ipc":588,"inventors":589,"tags":590,"tagNames":591},"gpi_00997","串级回旋加速器与双冷却储存环系统","本发明涉及一种串级回旋加速器与双冷却储存环系统。包括有离子源(500)和对称磁聚焦结构的冷却储存环(300)，对称磁聚焦结构的冷却储存环(300)注入端通过第一离子束输送线(600)与扇聚焦回旋加速器(100)或\u002F和分离扇聚焦回旋加速器(200)相接，对称磁聚焦结构的冷却储存环(300)引出端通过第二离子束输送线(700)与非对称磁聚焦结构的冷却储存环(400)相接。其采用一台或多台回旋加速器的级联组合、一个用于提高离子束流品质的带离子冷却技术装置的同步加速器储存环，同样也采用离子冷却技术装置的冷却储存环，有效满足高精度核物理试验及原子物理实验，并可应用于材料辐照、质子－重离子治癌等应用性研究项目。","中国科学院近代物理研究所","2010.01.20#公开;2010.03.24#实质审查的生效;2010.11.24#授权","CN200910000757.X","H05H13\u002F00;H05H13\u002F10;H05H7\u002F00;H05H7\u002F04","夏佳文;原有进",[],[],{"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1，其中VP3的基因序列为SEQ 3，其中VP1的基因序列为SEQ 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